Composite

Part:BBa_K3718012:Design

Designed by: Chan Pui Ching   Group: iGEM21_Hong_Kong_UCCKE   (2021-10-04)


Device for knocking out arcA and iclR genes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4357
    Illegal suffix found in sequence at 4527
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 4614
    Illegal SpeI site found at 4770
    Illegal PstI site found at 4784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4357
    Illegal EcoRI site found at 4599
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4320
    Illegal NheI site found at 4343
    Illegal NheI site found at 4562
    Illegal NheI site found at 4585
    Illegal SpeI site found at 4528
    Illegal SpeI site found at 4770
    Illegal PstI site found at 4542
    Illegal PstI site found at 4784
    Illegal NotI site found at 4363
    Illegal NotI site found at 4535
    Illegal NotI site found at 4605
    Illegal NotI site found at 4777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4357
    Illegal EcoRI site found at 4599
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4357
    Illegal suffix found in sequence at 4528
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 4614
    Illegal SpeI site found at 4770
    Illegal PstI site found at 4784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4357
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 4372
    Illegal XbaI site found at 4614
    Illegal SpeI site found at 4528
    Illegal SpeI site found at 4770
    Illegal PstI site found at 4542
    Illegal PstI site found at 4784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3573


Design Notes

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Source

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References